CA II Antibody (H-10)recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CA II Antibody (H-10): sc-133110

CA II Antibody (H-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • CA II Antibody (H-10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 191-260 of CA II of human origin
    • recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can CA II (H-10): sc-133110 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, CA II (H-10): sc-133110 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Robust signal in WB in mouse CA II transfected Robust signal in WB in mouse CA II transfected 293T: and HEL 92.1.7 (whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-01-26
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