CA II Antibody (G-2)recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CA II Antibody (G-2): sc-48351

CA II Antibody (G-2) is rated 4.8 out of 5 by 4.
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Datasheets

    • CA II Antibody (G-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 191-260 of CA II of human origin
    • CA II Antibody (G-2) is recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • CA II Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CA II (G-2): sc-48351.
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what is a recomended dilution for sc48351 AF 488 antibody to stain for FACS.

Asked by: angry Bird
Thank you for your question. We recommend using 1 µg of fluorochrome-conjugated antibody primary antibody, CA II (G-2): sc-48351 AF488 for each tube of 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. A link to our recommended protocol for flow cytometry is below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-21

Can CA II (G-2): sc-48351 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CA II (G-2): sc-48351 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from We used this antibody to visualize differentiated We used this antibody to visualize differentiated enterocytes on paraffin-embedded sections of human colonic epithelia. Signal specificity was great for doing double and triple stainings. Immunohistochemical staining on FFPE sections from human colonic tissue works also very nicely with this antibody.
Date published: 2016-05-31
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-03-21
Rated 4 out of 5 by from Shows good detection of endogenous and transfected Shows good detection of endogenous and transfected CA II in Western Blot -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Good results seen in IHC with paraffin Good results seen in IHC with paraffin-embedded mouse intestinal epithelium. -SCBT Publication Review
Date published: 2015-02-15
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