Anti-CA II Antibody (D-8) recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CA II Antibody (D-8): sc-133111

Anti-CA II Antibody (D-8) is rated 5.0 out of 5 by 5.
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Datasheets

    • CA II Antibody (D-8) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 191-260 of CA II of human origin
    • recommended for detection of CA II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • See CA II (G-2): sc-48351 for CA II antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CA II Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with CA II Antibody (D-8) (see ordering information below).
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Can CA II (D-8): sc-133111 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, CA II (D-8): sc-133111 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Positive signal in WB in human CA II transfected Positive signal in WB in human CA II transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Robust signal in WB in c4 whole cell lysate Robust signal in WB in c4 whole cell lysate and mouse kidney and rat kidney tissue extracts. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Good specificity in WB in human transfected Good specificity in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Worked in WB in mouse CA II transfected 293Tand Worked in WB in mouse CA II transfected 293Tand HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-22
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