Anti-CA I Antibody (F-5) recommended for detection of CA I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CA I Antibody (F-5): sc-393490

Anti-CA I Antibody (F-5) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-CA I Antibody (F-5) is a mouse monoclonal IgG2b κ CA I antibody, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 45-64 near the N-terminus of CA I (carbonic anhydrase) of human origin
    • Anti-CA I Antibody (F-5) is recommended for detection of CA I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
    • Anti-CA I Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CA I (F-5): sc-393490.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CA I Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with CA I Antibody (F-5) (see ordering information below).
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What application is the blocking peptide sc-393490 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great signal in WB I have used this antibody for hypoxia experiments. I obtained an excellent specific signal particularly in hypoxic cancer cells by western blotting. Recommend.
Date published: 2019-11-18
Rated 4 out of 5 by from Works well We have this antibody in our laboratory and works well.
Date published: 2017-01-23
Rated 5 out of 5 by from Nice Western Blot data of CA I expression in non Nice Western Blot data of CA I expression in non-transfected 293T, mouse CA I transfected 293T, HEL 92. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Nice Western Blot data of CA I expression in non Nice Western Blot data of CA I expression in non-transfected and human CA I transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-21
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