C5b-9 Antibody (2A1)recommended for detection of a neoantigen of the C5b-9 complex of mouse and rat origin by WB and IF

C5b-9 Antibody (2A1): sc-66190

C5b-9 Antibody (2A1) is rated 4.0 out of 5 by 3.
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Datasheets

    • C5b-9 Antibody (2A1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified C5b-9 of rat origin
    • recommended for detection of a neoantigen of the C5b-9 complex of mouse and rat origin by WB and IF
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C5b-9 (2A1): sc-66190.
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Are there any issues with using sc-66190: C5b-9 (2A1) mouse monoclonal primary antibody on human samples?For flow cytometer?

Asked by: Jingchao
Thank you for your question. C5b-9 Antibody (2A1): sc-66190 is recommended for detection of a neoantigen of the C5b-9 complex of mouse and rat origin by WB and IF. It is not recommended for detection of human C5b-9. The antibody has not been tested for flow cytometry and currently there is no data indicating whether or not the antibody is suitable for this application.
Answered by: Tech Support Europe
Date published: 2019-05-27

Are there any issues with using sc-66190: C5b-9 (2A1) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-66190 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Workded as expected. Worked well for IF staining in the in vitro bacterial killing experiment.
Date published: 2019-04-24
Rated 4 out of 5 by from 结果符合预期 以小鼠作为模式动物,流式检测补体系统在损伤的肝细胞表面的激活。 选择荧光直标抗体后,检测结果较好。 对于诱导损伤的肝细胞Necrosis-1和-2,均能检测到显著的C5b-9结合。
Date published: 2019-02-12
Rated 4 out of 5 by from Works well for IF applications Works well for IF applications, positive in guinea pig nerve samples. -SCBT Publication Review
Date published: 2015-02-03
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