C3 Antibody (C-4)recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α' chain of human origin by WB, IP, IF and ELISA

C3 Antibody (C-4): sc-25298

C3 Antibody (C-4) is rated 5.0 out of 5 by 3.
  • y_2019, m_11, d_15, h_12
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.2
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_25298, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 92ms
  • REVIEWS, PRODUCT
5
3
4
0
3
0
2
0
1
0

Datasheets

    • C3 Antibody (C-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 541-840 of C3 of human origin
    • recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α' chain of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See C3 (B-9): sc-28294 for C3 antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Submit a review for this product and receive 15 CruzCredits

What is the recommended fixation method for immunofluorescence staining with C3 (C-4): sc-25298 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
  • y_2019, m_11, d_15, h_12CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.2
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_25298, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 91ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Positive signal in WB in human transfected Positive signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Robust signal in WB in Hep G2 whole cell lysate Robust signal in WB in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-01-16
  • y_2019, m_11, d_15, h_12
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.2
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_25298, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.