C3 Antibody (B-9)recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α' chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

C3 Antibody (B-9): sc-28294

C3 Antibody (B-9) is rated 4.7 out of 5 by 16.
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Datasheets

    • C3 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 541-840 of C3 of human origin
    • C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α' chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C3 (B-9): sc-28294.
    • See all information
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Has anyone used this AB for IHC in rat brain tissue? 

Asked by: Anonymous
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
Answered by: Tech Service
Date published: 2019-08-14

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

Asked by: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
Answered by: Technical Support
Date published: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

Asked by: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
Answered by: Technical Support
Date published: 2018-12-11

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

Asked by: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-04-12

How can C3 Antibody (B-9): sc-28294 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-28294 PE), fluorescein (sc-28294 FITC), Alexa Fluor® 488 (sc-28294 AF488) or Alexa Fluor® 647 (sc-28294 AF647).
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from Works well 4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
Date published: 2018-08-01
Rated 1 out of 5 by from Does not detect human C3 in paraffin tissues I tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
Date published: 2017-05-08
Rated 5 out of 5 by from Great for all manner of C3 detection. This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
Date published: 2017-01-05
Rated 5 out of 5 by from Superb!!! Great western if and ihc results; strong signal and low background
Date published: 2017-01-05
Rated 4 out of 5 by from IHC-P some macrophages were positive for C3 by this antobody in IHC-P method
Date published: 2016-12-30
Rated 5 out of 5 by from Great in multiple applications This antibody works very well in Western Blot, IF and IHC(P)
Date published: 2016-12-23
Rated 5 out of 5 by from I used it to detect mouse complement C3b I used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
Date published: 2015-10-15
Rated 5 out of 5 by from Great stain in mouse intestinal tissues Great stain in mouse intestinal tissues.
Date published: 2015-08-06
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