C3 Antibody (A-4)recommended for detection of C3 of human origin by WB, IP, IF and ELISA

C3 Antibody (A-4): sc-55458

C3 Antibody (A-4) is rated 5.0 out of 5 by 4.
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Datasheets

    • C3 Antibody (A-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 541-840 of C3 of human origin
    • recommended for detection of C3 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See C3 (B-9): sc-28294 for C3 antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
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What is the recommended fixation method for immunofluorescence staining with C3 (A-4): sc-55458 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from ideal result I purchased this antibody in mid-May and received it in early June in China. I used it to identify C3 in the protein mixtures obtained from the early purifying stage by WB. After diluting to a larger ratio than the recommended value, I've got a clear and beautiful strip appearing its beta chain after reducing SDS-PAGE.
Date published: 2017-07-27
Rated 5 out of 5 by from Great Western blot data obtained using HepG2 Great Western blot data obtained using HepG2 samples. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Worked well for human transfected lysates Worked well for human transfected lysates. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-04
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