C3 Antibody (2898)recommended for detection of the C-terminus of C3a and intact C3 of human origin by WB and IP

C3 Antibody (2898): sc-52629

C3 Antibody (2898) is rated 4.0 out of 5 by 1.
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Datasheets

    • C3 Antibody (2898) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against the C-terminus of C3 of human origin
    • recommended for detection of the C-terminus of C3a and intact C3 of human origin by WB and IP
    • See C3 (B-9): sc-28294 for C3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using C3 (2898): sc-52629 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from WB: Great data obtained using HepG2 samples WB: Great data obtained using HepG2 samples and human transfected cell lysates. -SCBT QC
Date published: 2014-03-02
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