C2 Antibody (E-7)recommended for detection of C2 of human origin by WB, IP, IF, IHC(P) and ELISA

C2 Antibody (E-7): sc-373809

C2 Antibody (E-7) is rated 5.0 out of 5 by 2.
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Datasheets

    • C2 Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 204-503 mapping within an internal region of C2 of human origin
    • C2 Antibody (E-7) is recommended for detection of C2 of human origin by WB, IP, IF, IHC(P) and ELISA
    • C2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C2 (E-7): sc-373809.
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Can it be used to detect pig-derived C2 by WB or ELISA?

Asked by: LIZ2017
Thank you for your question. We have not tested this antibody, C2 (E-7): sc-373809, for detection of C2 in pig samples and so this species is not recommended at this time. This monoclonal antibody is raised against amino acids 204-503 mapping within an internal region of C2 of human origin. Based on sequence analysis, this is predicted to share 85% sequence identity with a published C2 protein sequence of pig origin (accession number NP_001095285). This is below our threshold to recommend the antibody for use in pig, however the identity is high enough where there is a good chance that the antibody will also recognize the C2 protein in pig.
Answered by: Technical Support
Date published: 2017-09-15

Can C2 (E-7): sc-373809 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, C2 (E-7): sc-373809 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced great immunoperoxidase membrane Produced great immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Good Western Blot data of C2 expression in Good Western Blot data of C2 expression in human liver, human spleen and human ovary tissue extracts. -SCBT QC
Date published: 2014-01-22
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