Anti-C1s Antibody (D-6) recommended for detection of mature C1 esterase and C1s precursor of human origin by WB, IP, IF and ELISA

Anti-C1s Antibody (D-6): sc-365273

Anti-C1s Antibody (D-6) is rated 4.8 out of 5 by 6.
  • y_2022, m_7, d_1, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_365273, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 88ms
  • REVIEWS, PRODUCT
loading
5
5
4
1
3
0
2
0
1
0

Datasheets

    • Anti-C1s Antibody (D-6) is a mouse monoclonal IgG2b κ C1s antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 140-208 mapping within an internal region of C1s of human origin
    • C1s Antibody (D-6) is recommended for detection of mature C1 esterase and C1s precursor of human origin by WB, IP, IF and ELISA
    • Anti-C1s Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C1s (D-6): sc-365273.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for C1s Antibody (D-6). This work is in progress.
loading

Hello, I'm testing mouse serums for c1s. I wonder if your D6 monoclonal (sc-365273) reacts to mouse c1s as well and whether you sell biotinylated version as well?

Asked by: IstvanBartok
Thank you for your question. We have not yet tested this antibody, C1s (D-6): sc-365273, for reactivity with C1s of mouse origin. We could provide this antibody conjugated to biotin as a special order. Please contact [email protected] for more information. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-10-31

What is the recommended starting dilution for Western blot for C1s Antibody (D-6): sc-365273?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended Western blot protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 15
Date published: 2017-01-24
  • y_2022, m_7, d_1, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_365273, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 93ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works well Works well in western blot of the ovine thymic tissue.
Date published: 2021-11-28
Rated 5 out of 5 by from Works fine in WB Works in human serum and fibroblast lysates in western blot
Date published: 2018-05-30
Rated 4 out of 5 by from WB There was a weak 90kd band in thp1 samples by WB..
Date published: 2017-01-19
Rated 5 out of 5 by from Excellent antibody C1s Antibody (D-6) is an excellent replacement for polyclonal C1s with all around better performance in WB and IF applications.
Date published: 2017-01-04
Rated 5 out of 5 by from Produced exceptional immunofluorescence cell Produced exceptional immunofluorescence cell surface staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Satisfactory Western Blot data of C1s expression Satisfactory Western Blot data of C1s expression in non-transfected, human C1s transfected 293T whole cell lysates and human platelet extract . -SCBT QC
Date published: 2013-03-19
  • y_2022, m_7, d_1, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.32
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_365273, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT