Anti-Myc/c-Myc Antibody (Myc.A7) recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of human and monkey origin by WB, IP and IF

Anti-Myc/c-Myc Antibody (Myc.A7): sc-56634

Anti-Myc/c-Myc Antibody (Myc.A7) is rated 4.3 out of 5 by 3.
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Datasheets

    • Myc/c-Myc Antibody (Myc.A7) is a mouse monoclonal IgG1, cited in 14 publications, provided at 100 µg/ml
    • raised against amino acids 410-419 of c-Myc of human origin
    • recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of human and monkey origin by WB, IP and IF
    • See Myc/c-Myc (9E10): sc-40 for Myc/c-Myc antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Myc/c-Myc Antibody (Myc.A7). This work is in progress.
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I am using c-Myc (Myc.A7): sc-56634 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from high titer diluted in 1:1000, and used for western blot, performed clean bands Hela cells.
Date published: 2016-11-04
Rated 4 out of 5 by from LnCaP LnCaP, 22rv1, DU145 and PC3, LAPC-4 and C4-2B cells in Western. -SCBT Publication Review
Date published: 2014-06-01
Rated 4 out of 5 by from Used at 1:250 in IF Used at 1:250 in IF. -SCBT Publication Review
Date published: 2011-12-01
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