Anti-Myc/c-Myc Antibody (C-8) recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA

Anti-Myc/c-Myc Antibody (C-8): sc-41

Anti-Myc/c-Myc Antibody (C-8) is rated 3.8 out of 5 by 10.
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_en_US, sid_41, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 153ms
  • REVIEWS, PRODUCT
loading
5
4
4
4
3
0
2
0
1
2

Datasheets

    • Myc/c-Myc Antibody (C-8) is a mouse monoclonal IgG2a κ, cited in 40 publications, provided at 200 µg/ml
    • raised against full length c-Myc of human origin
    • Anti-Myc/c-Myc Antibody (C-8) is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA
    • Anti-Myc/c-Myc Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-41 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Myc/c-Myc (C-8): sc-41.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Myc/c-Myc Antibody (C-8) for WB applications. These reagents are now offered in bundles with Myc/c-Myc Antibody (C-8) (see ordering information below).
loading

Could I use this to detect endogenous c-Myc in mouse cells by flow cytometry? If not, which antibody do you recommend?

Asked by: Mariaaaaaa
Yes you can. We offer this antibody conjugated to either phycoerythrin or FITC for IF, IHC(P) and Flow Cytometry.
Answered by: Dallas Tech Service
Date published: 2021-04-07

When should the TransCruz reagent sc-41 X be used instead of the standard form sc-41?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_12, d_6, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_41, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from 検出できません。 HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できませんでした。他社抗体では検出できます。
Date published: 2020-10-15
Rated 5 out of 5 by from Very good antibody I used this antibody to detect transfected mouse c-Myc in MEF cell line, and it works well at 1:1000 dilution for western blot and 1:100 for imunofluorescence. I think that for immunofluorescence greater dilutions might also work well.
Date published: 2019-10-04
Rated 5 out of 5 by from Gives clear bands Bands show up nice and clearly. Used a 1:1000 dilution for the WB
Date published: 2019-03-21
Rated 4 out of 5 by from GOOD It worked well in 1:3000 in our stable HEK293t cell lines
Date published: 2018-04-03
Rated 1 out of 5 by from knockin mouse embryo IP 2ug of Ab from sample failed to IP myc-tag protein. 9E10 and 9E11 worked fine
Date published: 2017-12-27
Rated 4 out of 5 by from Buena resolución En uso para WB a una concentración de 1:1000 obtenemos una banda única.
Date published: 2017-10-10
Rated 5 out of 5 by from WB detection We use it for WB detection.rat brain shows positive band at 67kda.The loading amount at 60ug. Strong signal for different dilutions from 1:500 to 1:1000.
Date published: 2017-09-16
Rated 5 out of 5 by from strong band c-Myc antibody detected in Western blot, 1:1000 been used, the band is unique
Date published: 2017-04-25
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_en_US, sid_41, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT