Anti-Myc/c-Myc Antibody (C-33) recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA

Anti-Myc/c-Myc Antibody (C-33): sc-42

Anti-Myc/c-Myc Antibody (C-33) is rated 4.3 out of 5 by 18.
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Datasheets

    • Myc/c-Myc Antibody (C-33) is a mouse monoclonal IgG1 κ, cited in 331 publications, provided at 200 µg/ml
    • raised against full length c-Myc of human origin
    • Anti-Myc/c-Myc Antibody (C-33) is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA
    • Anti-Myc/c-Myc Antibody (C-33) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-42 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Myc/c-Myc (C-33): sc-42.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Myc/c-Myc Antibody (C-33) for WB applications. These reagents are now offered in bundles with Myc/c-Myc Antibody (C-33) (see ordering information below).
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Is this antibody suitable for detection of mouse c-Myc? I am planning to overexpress mouse c-Myc in mouse embryonic fibroblast cell line.

Asked by: IO11
Thank you for your question. c-Myc Antibody (C-33): sc-42 is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2019-09-02

Can this antibody detect endogenous c-Myc protein as well as fusion proteins tagged with c-Myc?

Asked by: AbPolly
Thank you for your question. Yes, our c-Myc sc-42 is reactive with both endogenous c-Myc in samples, and with c-Myc tagged proteins in multiple applications.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 5 out of 5 by from 抗体 用了好几家抗体,Santa一如既往的好。这个抗体效果太棒了,WB条带特别漂亮,价格也比较合理,推荐大家使用和购买
Date published: 2020-11-28
Rated 1 out of 5 by from 検出できません。 HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できませんでした。他社抗体では検出できます。
Date published: 2020-10-15
Rated 5 out of 5 by from Gives a clear band at around 55 kDa Good antibody. We use a 1:1000 dilution for the western blot.
Date published: 2019-03-21
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 1 out of 5 by from knockin mouse embryo IP 2ug of Ab from sample failed to IP myc-tag protein. 9E10 and 9E11 worked fine.
Date published: 2017-12-27
Rated 2 out of 5 by from non specific bands I see lots of non specific bands when I try to detect endogenous Myc in U251 cells(1:200) and do not know which is the right band.
Date published: 2017-11-21
Rated 5 out of 5 by from Works very well The antibody worked great for us under the following conditions: 5% milk, 1:500 dilution, HEK whole cell lysates with RIPA lysis buffer. It is recommended for the detection of endogenous c-Myc
Date published: 2017-10-05
Rated 5 out of 5 by from Nice Western data Works better than my 9E10 antibody! very little background.
Date published: 2017-10-03
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