Anti-Myc/c-Myc Antibody (9E11) recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and monkey origin by WB, IP, IF and IHC(P)

Anti-Myc/c-Myc Antibody (9E11): sc-47694

Anti-Myc/c-Myc Antibody (9E11) is rated 4.0 out of 5 by 9.
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Datasheets

    • Myc/c-Myc Antibody (9E11) is a mouse monoclonal IgG2a κ, cited in 94 publications, provided at 200 µg/ml
    • raised against amino acids 408-420 within the C-terminal domain of c-Myc of human origin
    • Anti-Myc/c-Myc Antibody (9E11) is recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and monkey origin by WB, IP, IF and IHC(P)
    • Anti-Myc/c-Myc Antibody (9E11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to TRITC for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47694 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Myc/c-Myc (9E11): sc-47694.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Myc/c-Myc Antibody (9E11) for WB and IHC(P) applications. These reagents are now offered in bundles with Myc/c-Myc Antibody (9E11) (see ordering information below).
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What is the band size expected in western blotting when using 9E11 c-Myc antibody?

Asked by: priyanka2pdm
Thank you for your question. The expected molecular weight for endogenous c-myc is ~67kDa.
Answered by: Tech Service
Date published: 2019-02-12

When should the TransCruz reagent sc-47694 X be used instead of the standard form sc-47694?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 10
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with c-Myc (9E11): sc-47694?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by from Great signal rimary antibody c-Myc (9E11) sc-47694 was used diluted 1:1000 according to the manufacturer's recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membranes of PVDF have been incubated overnight at 4°C and then they have been probed for 1h at RT with secondary antibodies diluted 1:5000 in Milk 5%. We have tested this antibodiy three tumor cell lines: HeLa, A375 and glioblostoma primary cell line. In all the samples the recognition of the TAG was effective. The signal was clear and good, so we can conclude that the antibody has worked well. very well.
Date published: 2020-11-09
Rated 1 out of 5 by from バックが高い HEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できますが、他社より感度が低く、バックグラウンドシグナルが非常に多いです。
Date published: 2020-10-15
Rated 5 out of 5 by from Great antibody I ordered this antibody as a sample and tested it on A549, HEK293 and HeLa cell lines and had great bands.
Date published: 2020-05-10
Rated 3 out of 5 by from Antibody review Worked in WB with mouse brain tissue at 1:500 dilution.
Date published: 2018-05-29
Rated 4 out of 5 by from knockin mouse embryo IP 2ug of 9e11 works fine (slightly better than 9E10), but overall enrichment was not great compared to input.
Date published: 2017-12-27
Rated 5 out of 5 by from Outstanding product !! Very clear western blot data;also great for IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by from Good antibody for c-Myc Well-published antibody for c-Myc and c-Myc tagged fusion proteins. Strong, sensitive detection in Western blot and staining.
Date published: 2017-01-27
Rated 4 out of 5 by from Works in Western with human Ishikawa endometrial Works in Western with human Ishikawa endometrial cancer cell line. -SCBT Publication Review
Date published: 2014-01-01
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