Anti-Myc/c-Myc Antibody (1.N.2) recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP and IF

Anti-Myc/c-Myc Antibody (1.N.2): sc-70469

Anti-Myc/c-Myc Antibody (1.N.2) is rated 4.7 out of 5 by 3.
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Datasheets

    • Myc/c-Myc Antibody (1.N.2) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
    • raised against full length c-Myc of human origin
    • recommended for detection of c-Myc p67 and c-Myc tagged fusion proteins of mouse, rat, human and avian origin by WB, IP and IF
    • See Myc/c-Myc (9E10): sc-40 for Myc/c-Myc antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Myc/c-Myc Antibody (1.N.2). This work is in progress.
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What blocking solution should I use with c-Myc (1.N.2): sc-70469 monoclonal antibody for Western blot?

Asked by: cjMara
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from good results with this ab Pancreas correspond to Ela::Myc transgenic mice, which express c-Myc under pancreatic acinar cell Elastase promoter. Also works with wt mice, although at 1/50 dilution.
Date published: 2017-01-24
Rated 5 out of 5 by from great I used this production to analyze the expression of c-Myc in T47D cells by WB. The band was pretty bright.
Date published: 2016-12-05
Rated 4 out of 5 by from Good detection of both transfected and endogenous Good detection of both transfected and endogenous proteins in Western Blot. -SCBT QC
Date published: 2015-03-16
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