c-Jun Antibody (G-4) recommended for detection of c-Jun p39 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

c-Jun Antibody (G-4): sc-74543

c-Jun Antibody (G-4) is rated 4.5 out of 5 by 40.
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Datasheets

    • Anti-c-Jun Antibody (G-4) is a mouse monoclonal IgG1 (kappa light chain) c-Jun antibody provided at 200 µg/ml
    • raised against amino acids 1-79 of c-Jun of human origin
    • Anti-c-Jun Antibody (G-4) is recommended for detection of c-Jun p39 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-c-Jun Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-74543 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of c-Jun (G-4): sc-74543.
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Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-74543 PE), fluorescein (sc-74543 FITC), Alexa Fluor® 488 (sc-74543 AF488) or Alexa Fluor® 647 (sc-74543 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 14
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 5 out of 5 by from SDSPAGE Single and clear band in human cell line during ip 1:1000
Date published: 2018-06-13
Rated 2 out of 5 by from Not good This one worked as worse as the previous tested samples, so I will not recommend it.
Date published: 2018-03-30
Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 4 out of 5 by from Good, but needs antigen retrieval We used this antibody on rat DRG tissue, it worked great at 1:200, but needs citrate antigen retrieval, or it won't work.
Date published: 2017-11-29
Rated 1 out of 5 by from Didnot work for WB Did not work for western blot on cultured cells on dish
Date published: 2017-11-21
Rated 1 out of 5 by from Not working in Rat sciatic nerves (WB) Received as a free sample Test with 20µg Rat sciatic nerves (CelLytic™ MT Cell Lysis Reagent ) Blocking buffer: TBST-BSA 5% Incubation: 1:1000 in TBST-BSA 5% No band are detected around the expected size.
Date published: 2017-11-20
Rated 5 out of 5 by from Not bad Pretty good for detect the expression of c-Jun level in HepG2.2.15 at 1:500 dilution.
Date published: 2017-10-07
Rated 4 out of 5 by from 抗体特异性好 该试用装抗体的使用效果非常好,条带单一且非常清晰,没有过多的杂带,背景也不是很差,希望公司继续努力。
Date published: 2017-09-08
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