c-IAP1 Antibody (F-4)recommended for detection of c-IAP1 of human origin by WB, IP, IF and ELISA

c-IAP1 Antibody (F-4): sc-271419

c-IAP1 Antibody (F-4) is rated 4.4 out of 5 by 14.
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Datasheets

    • c-IAP1 Antibody (F-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 111-193 mapping within an internal region of c-IAP1 (inhibitor of apoptosis protein 1) of apoptosis protein 1 of human origin
    • c-IAP1 Antibody (F-4) is recommended for detection of c-IAP1 of human origin by WB, IP, IF and ELISA
    • c-IAP1 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of c-IAP1 (F-4): sc-271419.
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What is the recommended starting dilution for Western blot for c-IAP1 (F-4): sc-271419 monoclonal antibody?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-271419.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 4 out of 5 by from Good performance for WB! I tried 1:500 for cIAP1 overexpression in HEK293T cells and it works perfectly. The band is clean and sharp, without background.
Date published: 2017-09-28
Rated 5 out of 5 by from positive band I got the target bands at 70kda by WB,diluted the antibody 1:1000,Hela cell used for positive control.We got positive band.
Date published: 2017-09-03
Rated 5 out of 5 by from good for WB Tried 1:200 ,1:500, and 1:1000 dilution.I suggest use the dilution around 1:500,stong signal and clean and no background.
Date published: 2017-08-26
Rated 5 out of 5 by from Very clean result c-IAP1 the antibody is very suitable for me, I can even see good bands at a low diluted condition (1:2000), Very clean and stable bands without any background.
Date published: 2017-05-28
Rated 5 out of 5 by from perfect antibody Works perfectly in HeLa whole cell lysates, high recommend 1:500 dilution
Date published: 2017-04-26
Rated 5 out of 5 by from Great Antibody c-IAP1 antibody works perfectly with 293T cells for WB. The band is sharp and strong.
Date published: 2017-04-17
Rated 5 out of 5 by from good c-IAP1 antibody Very good c-IAP1 antibody for WB I suggest dilution this antibody at 1:800.
Date published: 2017-04-08
Rated 5 out of 5 by from Good Antibody c-IAP1 antibody worked well in the human samples. It had powerful band detected in a large number of cell lines, the result satisfied.
Date published: 2017-02-07
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