c-Fms/CSF-1R Antibody (D-8)recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

c-Fms/CSF-1R Antibody (D-8): sc-365719

c-Fms/CSF-1R Antibody (D-8) is rated 3.8 out of 5 by 5.
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Datasheets

    • c-Fms/CSF-1R Antibody (D-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 943-971 at the C-terminus of c-Fms/CSF-1R of human origin
    • c-Fms/CSF-1R Antibody (D-8) is recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • c-Fms/CSF-1R Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-365719 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of c-Fms/CSF-1R (D-8): sc-365719.
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Does this antibody block CSF-1R activation?

Asked by: karel
Thank you for your question. This antibody has not been tested for blocking applications, so we do not have any information regarding its use for that purpose. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-24

What application is the blocking peptide sc-365719 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from so, so this ab detects 3 bands, 70, 100, 130 kDa in breast and ovarian cancer cells.
Date published: 2017-04-21
Rated 5 out of 5 by from Published WB data with mouse samples Published WB data with mouse samples - SCBT Publication Review
Date published: 2015-04-29
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-02-23
Rated 3 out of 5 by from Robust band seen in WB using THP Robust band seen in WB using THP-1 whole cell lysate. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Produced beautiful immunofluorescence membrane Produced beautiful immunofluorescence membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2013-10-13
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