Anti-c-Fms/CSF-1R Antibody (B-8) recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-c-Fms/CSF-1R Antibody (B-8): sc-46662

Anti-c-Fms/CSF-1R Antibody (B-8) is rated 3.1 out of 5 by 8.
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Datasheets

    • Anti-c-Fms/CSF-1R Antibody (B-8) is a mouse monoclonal IgG1 λ c-Fms/CSF-1R antibody, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 11-310 of c-Fms/CSF-1R of human origin
    • Anti-c-Fms/CSF-1R Antibody (B-8) is recommended for detection of c-Fms/CSF-1R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-c-Fms/CSF-1R Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of c-Fms/CSF-1R (B-8): sc-46662.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for c-Fms/CSF-1R Antibody (B-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with c-Fms/CSF-1R Antibody (B-8) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 1 out of 5 by from this antibody detects too much non-specific bands I have used this ab for a few years. The ab purchased last year was good. But recent purchase (February 2021) is not good any more. It detects too many non-specific bands. The monoclonal hybridoma cells etc.. may be not good any more. Can you send me other lot of this ab? I attach westerns from 2020 and 2021 Feb with two different lots of c-fms ab.
Date published: 2021-02-17
Rated 1 out of 5 by from Did not function For this moment, I did not arrive to find band with this antibody. Must try other conditions. I use the antibody at 1:400 on primary human macrophages.
Date published: 2017-10-17
Rated 1 out of 5 by from Keine Ergebnisse in IF oder IHC Färbung Wir haben den Antikörper im Gewebe mit und ohne Citratbehandlung ausprobiert. Er funktioniert weder bei Immunfluoreszenz- noch IHC-Färbungen. Außerdem haben wir ihn auf Zellen ausprobiert und auch hier keine Ergebnisse erhalten.
Date published: 2017-03-31
Rated 5 out of 5 by from Great Antibodies We bought this antibody in September and it works great every times in our WB
Date published: 2017-03-02
Rated 5 out of 5 by from Good staining This antibody gave us a good IP staining on human tissue
Date published: 2016-12-20
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-08
Rated 4 out of 5 by from Nice band detected in WB using human recombinant c Nice band detected in WB using human recombinant c-Fms/CSF-1R. -SCBT QC
Date published: 2015-01-18
Rated 3 out of 5 by from Robust bands seen in WB using THP Robust bands seen in WB using THP-1 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-01-15
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