Anti-c-Fms/CSF-1R Antibody (2-4A5) recommended for detection of c-Fms gp130 and gp150 of human origin by WB and IP

Anti-c-Fms/CSF-1R Antibody (2-4A5): sc-01

Anti-c-Fms/CSF-1R Antibody (2-4A5) is rated 4.8 out of 5 by 8.
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Datasheets

    • c-Fms/CSF-1R Antibody (2-4A5) is a rat monoclonal IgG1, cited in 11 publications, provided at 200 µg/ml
    • raised against the cell surface (extracellular) epitope of c-Fms/CSF-1R of human origin
    • c-Fms/CSF-1R Antibody (2-4A5) is recommended for detection of c-Fms gp130 and gp150 of human origin by WB and IP
    • Anti-c-Fms/CSF-1R Antibody (2-4A5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of c-Fms/CSF-1R (2-4A5): sc-01.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for c-Fms/CSF-1R Antibody (2-4A5). This work is in progress.
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If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: TinTin
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support 14
Date published: 2017-01-22
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Rated 5 out of 5 by from Great western data Very clean western blot data; strong signal, low background
Date published: 2017-02-05
Rated 5 out of 5 by from Excellent antibody! c-Fms/CSF-1R Antibody (2-4A5) is a number one choice for your research applications!
Date published: 2016-12-28
Rated 5 out of 5 by from Worked great for Flow Cytometry on mouse Worked great for Flow Cytometry on mouse transfetced bone marrow mononuclear cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Great WB data obtained Great WB data obtained. Great antibody for this application. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Good data with paraffin Good data with paraffin-embedded human cervix carcinoma. -SCBT Publication Review
Date published: 2015-03-25
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP with human bone marrow cells. -SCBT Publication Review
Date published: 2015-03-23
Rated 4 out of 5 by from Published data for Flow Cytometry for use Published data for Flow Cytometry for use in PBM perepheral blood monocytes -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Published good quality flow cytometry data Published good quality flow cytometry data in human G1E-ER2. -SCBT Publication Review
Date published: 2013-09-13
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