C-6 NBD ceramide is a fluorescent sphingolipid analogue that is permeable to cell membranes and is a vital stain for the Golgi apparatus, endoplasmic reticulum, and nuclear envelope of mammalian cells. In the presence of 10 microM monensin (sc-200109) the visibility of the fluorescence is diminished on the plasma membrane, whereas the appearance of the Golgi apparatus is amplified. C-6 NBD ceramide has been known to produce the two fluorescent metabolites sphingomyelin and glucocerebroside intracellularly in the Golgi apparatus, which are then transported to the cell surface. In addition, monensin inhibits the ability to remove the fluorescent metabolites sphingomyelin and glucocerebroside from the cell surface by back exchange.
1. Lipsky, N.G. and Pagano, R.E. 1985. J. Cell Biol. 100: 27-34. PMID: 3965473 2. Lanfredi-Rangel, A., et al. 1999. FEMS Microbiol. Lett. 181: 245-251. PMID: 10585545 3. Li, R., et al. 1999. J. Biol. Chem. 274: 21121-21127. PMID: 10409665
Soluble in ethanol (~10 mg/ml), methanol (~10 mg/ml), chloroform (~10 mg/ml), and DMSO (~10 mg/ml).
Store at -20° C
n20D 1.56 (Predicted)
λex 467 nm, λem 535 nm in methanol
pKa: 13.54 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of
van IJzendoornvan IJzendoorn, SC. et al. (PubMed 9128247) utilized C-6 NBD ceramide, a known fluorescent sphingolipid analogue, to determine if glucosylceramide and sphingomyelin demonstrate a preferential localization in HepG2 cells. -SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.