C-12 NBD-dihydro-Ceramide

C-12 NBD-dihydro-Ceramide (CAS 474943-05-0)

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Synonym: N-C12-NBD-D-erythro-dihydro-Sphingosine
Application: A fluorescent ceramidase substrate
CAS Number: 474943-05-0
Molecular Weight: 661.9
Molecular Formula: C36H63N5O6
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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C-12 NBD-dihydro-Ceramide compound is a fluorescent ceramide analog which contains a saturated bond in the C4-C5 position of the sphingosine backbone. C-12 NBD ceramide, is a fluorescent ceramidase substrate which can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources. C-12 NBD-dihydro-ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide. This is based on the observation that saturation of the C4-C5 sphingosine bond in C16-ceramide results in approximately a 10-fold reduction in efficiency as a substrate for rat brain ceramidase compared to the unsaturated substrate. Yet, experimental characterization of C-12 NBD-dihydro-ceramide as a ceramidase substrate needs to be performed.


References

1 Tani, M., Okino, N., Mori, K., et al. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem 275(15) 11229-11234 (2000). 2 Ferlinz, K., Kopal, G., Bernardo, K., et al. Human acid ceramidase. Processing, glycosylation, and lysosomal targeting. J Biol Chem 276 (38) 35352-35360 (2001). 3 Tani, M., Okino, N., Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J Biochem 125 746-749 (1999). 4 Bawab, S.E., Usta, J., Roddy, P., et al. Substrate specificty of rat brain ceramidase. J Lipid Res 43 141-148 (2002).

Formulation :
A solution in chloroform:ethanol (1:1)
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Transport :
UN 1992, Class 3(6.1), Packing group II
PubChem CID :
53394579
SMILES :
CCCCCCCCCCCCCCCC(C(CO)NC(=O)CCCCCCCCCCCNC1=CC=C(C2=NON=C12)[N+](=O)[O-])O

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Is it possible to detect your product (sc-221389) C-12 NBD-dihydro-Ceramide on TLC plates by Fluorescense?

Asked by: Alexey
Thank you for your question. According to the literature, it is possible to detect this research chemical, C-12 NBD-dihydro-Ceramide, using TLC by fluorescence. However, we have not performed this experiment with the chemical ourselves, and so we are not able to provide a protocol. We recommend consulting the literature to see how others have used this chemical in TLC research applications.
Answered by: Technical Support
Date published: 2016-12-07
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