BTBD1 Antibody (A-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-60 mapping at the N-terminus of BTBD1 of human origin
BTBD1 Antibody (A-12) is recommended for detection of BTBD1 of human origin by WB, IP, IF and ELISA
BTBD1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BTBD1 (A-12): sc-398235.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
BTBD1 (BTB/POZ domain containing 1), also known as NS5ATP8 (hepatitis C virus (HCV) NS5A-transactivated protein 8), is a ubiquitously expressed cytoplasmic protein with predominant expression in skeletal muscle and heart. BTBD1 is expressed in a wide variety of species ranging from C. elegans to humans and specifically localizes to cytoplasmic bodies. It shares 80% amino acid sequence identity with its homolog, BTBD2. BTBD1 contains one BTB/POZ domain, which is a hydrophobic-rich motif known to mediate homomeric and heteromeric POZ-POZ interactions that are commonly found at the N-terminus of developmentally regulated zinc-finger transcription factors. BTBD1 also contains a C-terminal kelch-like region and a PHR-like region. Via its C-terminus, BTBD1 is capable of interacting with Topo I. In addition, BTBD1 is required for myoblast growth and differentiation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with BTBD1 (A-12): sc-398235 monoclonal antibody?
Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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