Bromobimane is a fluorescent probe that readily reacts with low molecular weight and protein thiols. It is almost non-fluorescent until it is conjugated to thiol. The reagent is particularly useful for quantifying glutathione.
Kosower, N.S., et al. Proc. Natl. Acad. Sci. USA 76, 3382, (1979); Danielsohn, P. and Nolte, A. Histochemistry 86, 281, (1987)
Soluble in water (partly), DMSO, DMF, methanol (25 mg/ml), and chloroform.
Store at -20° C
~327.8° C at 760 mmHg (Predicted)
~1.7 g/cm3 (Predicted)
n20D 1.64 (Predicted)
λex 384 nm, λem 462 nm in methanol; λex 390 nm, λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione); λex 398 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Certificate of Analysis
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Rated 5 out of
Ju et alJu et al. (PubMed ID 19394283) used Bromobimane to visualize 2,3-dimercaptosuccinic acid in mice blood and tissues via HPLC using fluorescence. -SCBT Publication Review
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