BrdU Antibody (MoBU-1)recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM

BrdU Antibody (MoBU-1): sc-51514

BrdU Antibody (MoBU-1) is rated 5.0 out of 5 by 5.
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Datasheets

    • BrdU Antibody (MoBU-1) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against 5-bromodeoxyuridine (BrdU) conjugated to hemocyanine
    • recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM
    • recognizes BrdU in denatured DNA of cells labeled with BrdU
    • suggested companion product: 5-Bromo-2′ deoxyuridine: sc-290815
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BrdU (MoBU-1): sc-51514.
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Is it absolutely necessary to do a denaturing step to visualize the antibody by IF in paraffin sections? By treating the sections with 2M HCl, I am unable to see a proper DAPI staining and therefore cannot quantify the PERCENTAGE of BrdU positive cells.

Asked by: Mirabai
Thank you for your message. Please find on the following link our highly recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you have further questions, please do not hesitate to contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

What is the total volume provided of BrdU (MoBU-1): sc-51514 monoclonal antibody?

Asked by: cjMara
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-12-04
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Rated 5 out of 5 by from Reliable Antibody, Great for IHC This antibody is great for producing images of dividing cells. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-18
Rated 5 out of 5 by from useful in scientific research i bought BrdU antibody for my WB experiment ,it makes good result ,i recommand it
Date published: 2016-12-05
Rated 5 out of 5 by from IHC publishable data on rat sciatic nerve IHC publishable data on rat sciatic nerve - SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from Successfully used in Flow Cytometry with Successfully used in Flow Cytometry with human HCT116 cell - SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Publishable IHC (P) data with mouse adrenal Publishable IHC (P) data with mouse adrenal glands - SCBT Publication Review
Date published: 2013-02-09
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