BrdU Antibody (IIB5)recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM

BrdU Antibody (IIB5): sc-32323

BrdU Antibody (IIB5) is rated 4.5 out of 5 by 27.
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Datasheets

    • BrdU Antibody (IIB5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Bromodeoxyuridine (BrdU) conjugated to BSA
    • BrdU Antibody (IIB5) is recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM
    • recognizes BrdU in denatured DNA of cells labeled with BrdU; may cross-react with iododeoxyuridine (IrdU)
    • BrdU Antibody (IIB5) is available conjugated to agarose for IP; HRP for IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BrdU (IIB5): sc-32323.
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Can you use Trizol to isolate nucleic acids from agarose conjugated antibodies?

Asked by: GmoFlo
Thank you for your question. TRIzol is a biochemical used in the extraction of DNA, RNA, and proteins from cells,. We have not tried to isolate nucleic acids from AC conugated antibodies, so we are unable to provide this information.
Answered by: Tech Service
Date published: 2019-03-22

Does it react with Iododeoxyuridine (IdU)? I want to use it for recognizing IdU not BrdU.

Asked by: moz93
Thank you for your question. No, this antibody does not cross react with IdU.
Answered by: Technical Support
Date published: 2019-02-01

Hi Is this nuclease free? I want to use it for RNA pull down.

Asked by: ohsamboss
Thank you for your question. We do not routinely test for this and can not guarantee that the antibody in stock for our mouse monoclonal BrdU Antibody (IIB5): sc-32323 is nuclease free.
Answered by: Technical Support
Date published: 2017-02-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our new Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of binding protein conjugates is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody for BrdU is currently available conjugated to agarose (sc-32323 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32323 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32323 PE), fluorescein (sc-32323 FITC), Alexa Fluor® 488 (sc-32323 AF488) or Alexa Fluor® 647 (sc-32323 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2016-12-15
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Rated 5 out of 5 by from Worked excellent for immunoflorescence staining I got this as a sample product and I am happy it worked extremely well for standardizing my protocol.
Date published: 2019-03-22
Rated 5 out of 5 by from Great feature I bought this couple month ago and its working very nicely
Date published: 2018-06-08
Rated 5 out of 5 by from Great for co-IP It is a great antibody for co-IP, and I highly recommend it.
Date published: 2018-01-13
Rated 5 out of 5 by from Good detection of S phase in embryos We have used this antibody to detect BrDU incorporation during embryonic S phase in mouse two-cell embryos, using IF. We have obtained very good quality nuclear staining!
Date published: 2018-01-08
Rated 5 out of 5 by from Ottimo anticorpo Ho utilizzato questo anticorpo per l'immunoprecipitazione di RNA marcati con BrU è ottenuto un ottimo risultato, valutato sia per pcr convenzionale che real-time.
Date published: 2017-11-10
Rated 5 out of 5 by from Works very good on IF of tissue samples We used this product for the IF detection of BrdU labeled small intestine proliferative cells in the frozen sections. 1:200 dilution was used.
Date published: 2017-10-04
Rated 1 out of 5 by from BrdU beads quality varies lot to lot I have bought BrdU AC beads for 5 + years and approximately 50% of the lots do not IP efficiently. Santa Cruz needs to improve their quality control so we do not waste time and money with lot variability.
Date published: 2017-08-21
Rated 5 out of 5 by from Nice result Nice result used for WB test, band is clear and no background and non-specific bands.
Date published: 2017-06-15
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