BORIS Antibody (F-1)recommended for detection of BORIS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

BORIS Antibody (F-1): sc-377085

BORIS Antibody (F-1) is rated 4.5 out of 5 by 2.
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Datasheets

    • BORIS Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 91-210 mapping within an internal region of BORIS of human origin
    • BORIS Antibody (F-1) is recommended for detection of BORIS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • BORIS Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-377085 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BORIS (F-1): sc-377085.
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What is the recommended starting dilution for Western blot for sc-377085: BORIS (F-1) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from fine results I used this antibody for western, IF and IP. The antibody is fine for IF. Western is depend on cell line. Not always get clear bind in western. While the band is single.
Date published: 2017-10-11
Rated 5 out of 5 by from Nice Western blot data of BORIS expression Nice Western blot data of BORIS expression in human testis tissue extract. -SCBT QC
Date published: 2013-07-03
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