Blood Group Lewis a Antibody (SPM279) recommended for detection of Lewis a blood group antigen of human origin by WB, IP, IF and IHC(P)

Blood Group Lewis a Antibody (SPM279): sc-52988

Blood Group Lewis a Antibody (SPM279) is rated 4.0 out of 5 by 1.
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Datasheets

    • Blood Group Lewis a Antibody (SPM279) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against mucins isolated from ovarian cyst fluid of human origin
    • recommended for detection of Lewis a blood group antigen of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What blocking solution should I use with Blood Group Lewis a (SPM279): sc-52988 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Positive result in western blot analysis Positive result in western blot analysis of Blood Group Lewis a expression in WiDr whole cell lysate under non-reducing conditions. -SCBT QC
Date published: 2013-07-16
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