BIS-Tris PropaneCAS: 64431-96-5
MF: C11H26N2O6
MW: 282.4
A buffer that is useful in the pH 6.3-9.5 range.

BIS-Tris Propane (CAS 64431-96-5)

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Synonym: 1,3-bis[tris(Hydroxymethyl)methylamino]propane
Application: A buffer that is useful in the pH 6.3-9.5 range
CAS Number: 64431-96-5
Purity: ≥99%
Molecular Weight: 282.4
Molecular Formula: C11H26N2O6
* Refer to Certificate of Analysis for lot specific data (including water content).
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BIS-Tris Propane is a buffer that is useful in the pH 6.3 - 9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).


1. Weber, R. E. 1985. J Appl Physiol. 72(4): 1611-1615. PMID: 1592755

2. Eckert, K. A. and Kunkel, T. A. 1991. PCR Methods Appl. 1(1): 17-24. PMID: 1842916

3. Larsen, T. H., Et al. 2009. Macromolecules. 42(21): 8443-8450. PMID: 20161466

Appearance :
Crystalline powder
Physical State :
Solubility :
Soluble in water (428 mg/ml).
Storage :
Store at room temperature
Melting Point :
164-165° C (lit.)
Boiling Point :
~573.32° C at 760 mmHg (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.57 (Predicted)
pK Values :
pKa (25° C): pK1: 6.8, pK2: 9.0, pKb: 7.79 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
MDL Number :
EC Number :
Beilstein Registry :

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Certificate of Analysis

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I am learning about good buffers. This buffer has been listed as a zwitterionic buffer in many places. Your site is the only one ive seen, to list it as non-zwitterionic. Please advise.

Asked by: csquare
Thank you for your question. Thank you for noticing this error on our site, we have fixed this information. This buffer is zwitterionic.
Answered by: Tech Service
Date published: 2019-09-12
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