BIS-Tris Propane is a buffer that is useful in the pH 6.3 - 9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
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2. Eckert, K. A. and Kunkel, T. A. 1991. PCR Methods Appl. 1(1): 17-24. PMID: 1842916
3. Larsen, T. H., Et al. 2009. Macromolecules. 42(21): 8443-8450. PMID: 20161466
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