BID Antibody (E-7)recommended for detection of BID of mouse, rat and human origin by WB, IP, IF and ELISA

BID Antibody (E-7): sc-514622

BID Antibody (E-7) is rated 4.0 out of 5 by 1.
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Datasheets

    • BID Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 172-195 at the C-terminus of BID of human origin
    • BID Antibody (E-7) is recommended for detection of BID of mouse, rat and human origin by WB, IP, IF and ELISA
    • BID Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514622 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of BID (E-7): sc-514622.
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Is the Alexa-Fluor conjugate of this AB available for flow cytometry experiments as stated in the description or is it not, since the unconjugated AB is just recommended for WB, IP, IF & ELISA?

Asked by: Fest
Thank you for your question. We do not test all of our antibodies in flow cytometry. We recommend and warrant the Alexa Fluor® 488, 546, 594, and 647 conjugates of BID (E-7): sc-514622 for use in flow cytometry. You can find our recommended protocol for flow cytometry by following this link: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What application is the blocking peptide sc-514622 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Worked well! I used the antibody at a dilution of 1:500 in 5% milk/TBST and an incubation over the weekend for murin B16 melanoma cells and it worked quited specific.
Date published: 2018-02-26
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