Anti-beta 3 Tubulin Antibody (AA10) recommended for detection of β3 Tubulin of mouse, rat and human origin by WB, IP and IF

Anti-β3 Tubulin Antibody (AA10): sc-80016

Anti-β3 Tubulin Antibody (AA10) is rated 4.8 out of 5 by 4.
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Datasheets

    • β3 Tubulin Antibody (AA10) is a mouse monoclonal IgG2a κ, cited in 28 publications, provided at 200 µg/ml
    • raised against β3 Tubulin of rat origin, with epitope mapping to amino acids 436-450
    • Anti-beta 3 Tubulin Antibody (AA10) is recommended for detection of β3 Tubulin of mouse, rat and human origin by WB, IP and IF
    • Anti-beta 3 Tubulin Antibody (AA10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β3 Tubulin (AA10): sc-80016.
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I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 4 out of 5 by from Melanocortin 4 receptor activation protects striat α-Melanocyte stimulating hormone (α-MSH) is a melanocortin which exerts potent anti-inflammatory and antiapoptotic effects. Melanocortin 4 receptors (MC4R) are abundantly expressed in the brain and we previously demonstrated that [Nle(4), D-Phe(7)]melanocyte-stimulating hormone (NDP-MSH), an α-MSH analogue, increased expression of brain derived-neurotrophic factor (BDNF), and peroxisome proliferator-activated receptor- γ (PPAR-γ). We hypothesized that melanocortins could affect striatal cell survival through BDNF and PPAR-γ. First, we determined the expression of these factors in the striatum. Acute intraperitoneal administration (0.5 mg/kg) of α-MSH increased the levels of BDNF mRNA in rat striatum but not in rat cerebral cortex. Also, protein expression of PPAR-γ and MC4R was increased by acute treatment with α-MSH in striatum but not in cortex. No changes were observed by 48 h treatment. Next, we evaluated melanocortins effect on neuron and glial survival. 3-nitropropionic acid (3-NP), which is known to induce striatal degeneration, was used to induce cell death in the rat striatal cell line ST14A expressing mutant human huntingtin (Q120) or in ST14A cells expressing normal human huntingtin (Q15), in primary cultured astrocytes, and in BV2 cells. NDP-MSH protected Q15 cells, astrocytes and BV2 cells from death by 3-NP whereas it did not fully protect Q120 cells. Protection of Q15 cells and astrocytes was blocked by a MC4R specific inhibitor (JKC-363) and a PPAR-γ antagonist (GW9662). The BDNF receptor antagonist (ANA-12) abolished NDP-MSH protective effect in astrocytes but not in Q15 cells. We demonstrate for the first time that melanocortins, acting through PPAR-γ and BDNF, protect neurons and glial cells from 3-NP toxicity
Date published: 2018-12-13
Rated 5 out of 5 by from Very good result beta-3-tubulin antibody (AA10) sc-800166: Immunofluorescence staining of beta-3-tubulin (green) and S100 (red) in rat dorsal root ganglion fixed in 4% paraformaldehyde.
Date published: 2018-02-06
Rated 5 out of 5 by from Produced good western blot band The tubulin antibody produced a clear western blot band for our cell lysate
Date published: 2016-12-20
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong tubulin band detected in A-431, K-562 and PC-12 whole cell lysates. -SCBT QC
Date published: 2013-10-22
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