Anti-beta 2 Tubulin Antibody (7B9) recommended for detection of β2 Tubulin of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-β2 Tubulin Antibody (7B9): sc-47751

Anti-β2 Tubulin Antibody (7B9) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-β2 Tubulin Antibody (7B9) is a mouse monoclonal IgG1 κ β2 Tubulin antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 437-445 mapping at the C-terminus of β2A Tubulin of human origin
    • Anti-beta 2 Tubulin Antibody (7B9) is recommended for detection of β2 Tubulin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-beta 2 Tubulin Antibody (7B9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β2 Tubulin (7B9): sc-47751.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for β2 Tubulin Antibody (7B9) for WB and IHC(P) applications. This reagent is now offered in a bundle with β2 Tubulin Antibody (7B9) (see ordering information below).

Can β2 Tubulin (7B9): sc-47751 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, β2 Tubulin (7B9): sc-47751 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good antibody that works in IF with HeLa cells Good antibody that works in IF with HeLa cells. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from WB: Strong bands clean background with NIH/3T3 WB: Strong bands clean background with NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2014-09-15
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