Anti-beta Tubulin Antibody (TUB 2.1) recommended for detection of β Tubulin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)

Anti-β Tubulin Antibody (TUB 2.1): sc-58886

Anti-β Tubulin Antibody (TUB 2.1) is rated 4.8 out of 5 by 4.
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Datasheets

    • β Tubulin Antibody (TUB 2.1) is a mouse monoclonal IgG1 κ, cited in 25 publications, provided at 200 µg/ml
    • raised against purified brain β Tubulin of rat origin
    • Anti-beta Tubulin Antibody (TUB 2.1) is recommended for detection of β Tubulin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
    • Anti-beta Tubulin Antibody (TUB 2.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β Tubulin (TUB 2.1): sc-58886.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for β Tubulin Antibody (TUB 2.1) for WB and IHC(P) applications. These reagents are now offered in bundles with β Tubulin Antibody (TUB 2.1) (see ordering information below).

Can I use an anti mouse HRP conjugated secondary with this antibody?

Asked by: IAMLCN
Thank you for the question. This antibody is a mouse IgG. Yes, you can use anti-mouse secondary antibodies conjugated to -HRP for the detection of this primary antibody. We suggest using our m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) (sc-516102) which shows excellent and specific data in WB and IHC(P).
Answered by: Technical Support Europe
Date published: 2017-12-03

I am using β Tubulin (TUB 2.1): sc-58886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from perfect antibody Very good and highly sensitive antibody. Can be diluted 1/5000, so can perform many blots.
Date published: 2019-06-21
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of β Tubulin expression in NIH/3T3, A-431, KNRK and PC-12 whole cell lysates.
Date published: 2017-01-23
Rated 4 out of 5 by from IF: Decent cytoplasmic staining in HeLa samples IF: Decent cytoplasmic staining in HeLa samples. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Strong band detected at correct size in HeLa Strong band detected at correct size in HeLa. -SCBT QC
Date published: 2014-03-26
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