Anti-beta Tubulin Antibody (SAP.4G5) recommended for detection of β Tubulin of mouse, rat, human, Xenopus, avian, bovine and canine origin by WB, IP, IF and IHC(P)

Anti-β Tubulin Antibody (SAP.4G5): sc-58884

Anti-β Tubulin Antibody (SAP.4G5) is rated 5.0 out of 5 by 4.
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Datasheets

    • β Tubulin Antibody (SAP.4G5) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
    • raised against the C-terminus of β Tubulin isotype I of chicken origin
    • recommended for detection of β Tubulin of mouse, rat, human, Xenopus, avian, bovine and canine origin by WB, IP, IF and IHC(P)
    • See β Tubulin (D-10): sc-5274 for β Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using β Tubulin (SAP.4G5): sc-58884 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good antibody Good signal in WB with mouse adipocyte and liver tissue. Dilution 1:500. 20 µg protein.
Date published: 2017-08-20
Rated 5 out of 5 by from nice beta tubulin antibody We used this antibody for western blot in order to detect beta Tubulin expression. We got positive bands shown up at the expected molecular weight in HeLa cells.
Date published: 2017-01-27
Rated 5 out of 5 by from Good data obtained using antibody in paraffin Good data obtained using antibody in paraffin-embedded human fallopian tube. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Good Good, strong bands in WB with NIH/3T3, F9, A-431, BJAB, KNRK and HeLa whole cell lysates. Clean background. -SCBT QC
Date published: 2014-09-17
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