Anti-beta Tubulin Antibody (F-1) recommended for detection of β Tubulin of mouse, rat, human, Arabidopsis thaliana, Zea mays and Pisum sativum origin by WB, IP, IF and ELISA

Anti-β Tubulin Antibody (F-1): sc-166729

Anti-β Tubulin Antibody (F-1) is rated 4.5 out of 5 by 13.
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Datasheets

    • Anti-β Tubulin Antibody (F-1) is a mouse monoclonal IgG3 κ β Tubulin antibody, cited in 31 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 422-439 near the C-terminus of β Tubulin of Arabidopsis thaliana origin
    • Anti-beta Tubulin Antibody (F-1) is recommended for detection of β Tubulin of mouse, rat, human, Arabidopsis thaliana, Zea mays and Pisum sativum origin by WB, IP, IF and ELISA
    • Anti-beta Tubulin Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β Tubulin (F-1): sc-166729.

Hi, I am working on Mimulus, I would like to use a protein antibody as a reference for samples , I am wondering how to find out if the arabidopsis version works for mimulus plants? Thanks!

Asked by: bqing
Thank you for your question. You could get in touch with our technical service team to arrange a free sample of this antibody.
Answered by: Technical Support Europe
Date published: 2020-10-14

What application is the blocking peptide sc-166729 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 3
Date published: 2017-01-17
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Rated 3 out of 5 by from Buon funzionamento Ho comprato questo anticorpo un mese fa circa ed è stato testato 1:1000 su astrociti. Le bande sono intorno a 55kDa, ma il segnale è debole
Date published: 2021-03-17
Rated 5 out of 5 by from This antibody works beautifully! I ordered a free sample about a month ago. We tried it with Arabidopsis protein extract. It recognize Arabidopsis b-tubulin at 1:1000 dilution. There was no background signal.
Date published: 2018-03-08
Rated 3 out of 5 by from Nice Tubulin band, but with nonspecific bands Ran this antibody in Huh7 cells. Band at 55 kDa was nice and clear, but had two faint nonspecific bands at ~25 and 70 kDa.
Date published: 2017-12-15
Rated 4 out of 5 by from great I like this antibody as it has strong band binding and no background.
Date published: 2017-10-13
Rated 5 out of 5 by from Highly specific antibody in tobacco + arabidopsis Clear and highly specific band of medium strength in total leave extract from both tobacco (N. benthamiana) and Arabidopsis. The detection was mediated using a secondary antibody coupled to a near infra red fluorescent dye by LICOR.
Date published: 2017-10-10
Rated 4 out of 5 by from Great! It worked well for Hela cell samples and HEK293 cells
Date published: 2017-10-03
Rated 4 out of 5 by from Good quality The antibody gives good result for western blotting assays (dilution 1/500), good signal strength. We obtained two bands when tested tissue samples (mice liver). However, it was clear which band was the specific one.
Date published: 2017-09-12
Rated 5 out of 5 by from Great Antibody! I'm glad to use it and get good results with it in IF and WB.
Date published: 2017-04-24
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