Anti-beta Tubulin Antibody (3F3-G2) recommended for detection of all vertebrate forms of β Tubulin of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-β Tubulin Antibody (3F3-G2): sc-53140

Anti-β Tubulin Antibody (3F3-G2) is rated 4.8 out of 5 by 4.
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Datasheets

    • β Tubulin Antibody (3F3-G2) is a mouse monoclonal IgM κ, cited in 44 publications, provided at 200 µg/ml
    • raised against brain extract of rat origin
    • recommended for detection of all vertebrate forms of β Tubulin of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See β Tubulin (D-10): sc-5274 for β Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β Tubulin (3F3-G2): sc-53140.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for β Tubulin Antibody (3F3-G2) for WB and IHC(P) applications. This reagent is now offered in a bundle with β Tubulin Antibody (3F3-G2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am using β Tubulin (3F3-G2): sc-53140 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Nice fat band, but strong nonspecific band Ran this antibody in Huh7 cells. Got a nice robust band at 55 kDa, but had a pretty strong nonspecific band above 98 kDa.
Date published: 2017-12-15
Rated 5 out of 5 by from Great antibody The beta Tubulin Antibody (3F3-G2): sc-53140 works very well for IHC with formalin fixed, paraffin-embedded tissue sections showing staining with membrane and cytoplasmic localization. This antibody has also been cited over 10 times for further verification.
Date published: 2017-02-08
Rated 5 out of 5 by from Able to obtain very nice cytoplamic staining Able to obtain very nice cytoplamic staining in human paraffin sections. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Great data for human and rat Great data for human and rat, strong specific bands at correct size. -SCBT QC
Date published: 2014-01-25
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