Anti-beta Tubulin Antibody (2-28-33) recommended for detection of β Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P)

Anti-β Tubulin Antibody (2-28-33): sc-23949

Anti-β Tubulin Antibody (2-28-33) is rated 4.7 out of 5 by 7.
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Datasheets

    • β Tubulin Antibody (2-28-33) is a mouse monoclonal IgG1 κ, cited in 35 publications, provided at 200 µg/ml
    • raised against Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
    • Anti-beta Tubulin Antibody (2-28-33) is recommended for detection of β Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P)
    • Anti-beta Tubulin Antibody (2-28-33) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β Tubulin (2-28-33): sc-23949.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for β Tubulin Antibody (2-28-33) for WB and IHC(P) applications. These reagents are now offered in bundles with β Tubulin Antibody (2-28-33) (see ordering information below).

I am using β Tubulin (2-28-33): sc-23949 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works great in zebrafish Used in zebrafish ICC and it worked beautifully, used at 1:100, haven't used in whole mount yet but assuming it will work the same
Date published: 2018-02-26
Rated 4 out of 5 by from Worked for WB It gives good bands at 1:500 dilution for BEAS2B cells.
Date published: 2018-01-04
Rated 5 out of 5 by from Sucessful loading control This antibody always been used for loading control,I loading 20ug mouse brain tissue ,got strong band at 1:2000.
Date published: 2017-02-08
Rated 5 out of 5 by from Nice western blot results We used this antibody for a western blot experiment and got nice dark bands at the correct molecular weight
Date published: 2017-01-19
Rated 4 out of 5 by from Works well in Western Blot applications using Works well in Western Blot applications using A547 and UCI-107 cell lines.
Date published: 2015-05-11
Rated 5 out of 5 by from Specific and sensitive for WB Specific and sensitive for WB, works well in K-562. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Works well for immunofluorescence with HeLa Works well for immunofluorescence with HeLa samples. -SCBT QC
Date published: 2014-03-07
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