Anti-beta-glucuronidase Antibody (E-11) recommended for detection of β-glucuronidase of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-glucuronidase Antibody (E-11): sc-374629

Anti-β-glucuronidase Antibody (E-11) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-β-glucuronidase Antibody (E-11) is a mouse monoclonal IgG1 κ β-glucuronidase antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 352-651 mapping at the C-terminus of β-glucuronidase of human origin
    • beta-glucuronidase Antibody (E-11) is recommended for detection of β-glucuronidase of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta-glucuronidase Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-glucuronidase (E-11): sc-374629.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for β-glucuronidase Antibody (E-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with β-glucuronidase Antibody (E-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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鼠源的肝组织可以反应吗?

Asked by: irisrjy
感谢您的提问。β-glucuronidase Antibody (E-11): sc-374629 不推荐用于鼠的样本。Thank you for your question. β-glucuronidase Antibody (E-11): sc-374629 is not recommended for detection of mouse sample.
Answered by: Technical Support 8
Date published: 2018-12-21

I am using β-glucuronidase (E-11): sc-374629 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific detection in WB For IF in cold MeOH fixed cells and 4% PFA-fixed cells, I could not see obvious staining, but I detected specific a band by WB in over exposure. I think this antibody worked very well.
Date published: 2017-02-02
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-22
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