beta-casein Antibody (H-4)recommended for detection of β-casein of mouse and rat origin by WB, IP, IF and ELISA

β-casein Antibody (H-4): sc-166530

β-casein Antibody (H-4) is rated 4.3 out of 5 by 3.
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Datasheets

    • β-casein Antibody (H-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against a peptide mapping within an internal region of β-casein of mouse origin
    • beta-casein Antibody (H-4) is recommended for detection of β-casein of mouse and rat origin by WB, IP, IF and ELISA
    • beta-casein Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166530 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-casein (H-4): sc-166530.
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Is this antibody only recognizes secreted but not cytosolic b-casein when doing IHC? 

Asked by: onlyyui
Thank you for your question. Unfortunately I don't have that information, but I would predict that it can detect both if they are present in the sample. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-09-21

What application is the blocking peptide sc-166530 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from mouse mammary gland works well in Western blot experiment, dilution 1:200
Date published: 2017-02-20
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-06-28
Rated 5 out of 5 by from WB publishable data with human and mouse WB publishable data with human and mouse 293T cells and milk - SCBT Publication Review
Date published: 2013-10-03
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