Anti-beta-Arrestin-2 Antibody (H-9) recommended for detection of β-Arrestin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-β-Arrestin-2 Antibody (H-9): sc-13140

Anti-β-Arrestin-2 Antibody (H-9) is rated 4.6 out of 5 by 13.
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Datasheets

    • β-Arrestin-2 Antibody (H-9) is a mouse monoclonal IgG1 κ, cited in 90 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-33 at the N-terminus of β-Arrestin-2 of human origin
    • Anti-beta-Arrestin-2 Antibody (H-9) is recommended for detection of β-Arrestin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • available conjugated to phycoerythrin for IF, IHC(P) and FCM
    • beta-Arrestin-2 Antibody (H-9) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Arrestin-2 (H-9): sc-13140.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-Arrestin-2 Antibody (H-9) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Arrestin-2 Antibody (H-9) (see ordering information below).
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What is the recommended secondary reagent for Western blot in human samples?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 5 out of 5 by from Clean, strong signal Great antibody performance across the board in Western Blot, IF, IHC and Flow.
Date published: 2017-01-12
Rated 4 out of 5 by from WB: Worked well in rat AtT WB: Worked well in rat AtT-20. -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with HEY and OVCA 433 cells -SCBT Publication Review
Date published: 2015-04-05
Rated 5 out of 5 by from Strong signal achieved in WB with RAW 264 Strong signal achieved in WB with RAW 264.7 and PC-12 whole cell lysates. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human HEK293B2. -SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Antibody was published for use in Immunoprecipitation Antibody was published for use in Immunoprecipitation on embryonic kidney HEK293 cells. Worked well! -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Published data for IF in human SH Published data for IF in human SH-SY5Y cells -SCBT Publication Review
Date published: 2015-03-15
Rated 5 out of 5 by from Publishable Western blot results using MEFs Publishable Western blot results using MEFs. -SCBT Publication Review
Date published: 2015-03-11
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