Anti-beta-Arrestin-2 Antibody (B-4) recommended for detection of β-Arrestin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-Arrestin-2 Antibody (B-4): sc-365445

Anti-β-Arrestin-2 Antibody (B-4) is rated 4.8 out of 5 by 6.
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Datasheets

    • β-Arrestin-2 Antibody (B-4) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 385-408 at the C-terminus of β-Arrestin-2 of human origin
    • Anti-beta-Arrestin-2 Antibody (B-4) is recommended for detection of β-Arrestin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta-Arrestin-2 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Arrestin-2 (B-4): sc-365445.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for β-Arrestin-2 Antibody (B-4) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Arrestin-2 Antibody (B-4) (see ordering information below).
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What application is the blocking peptide sc-365445 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Endogenous Beta-Arrestin Detection Product sc-365445 was used at 1:600 ratio to detect endogenous beta-arrestin1 from HEK293T cells and revealed a unique band <50kDa. Immnohistochemistry resulted in a strong nuclear staining when used at 1:200 ratio in fixed HEK293T cells.
Date published: 2019-04-26
Rated 5 out of 5 by from Good for IF and IHC This β-Arrestin-2 antibody shows strong specific signal in IF and IHC stainings in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from Nice features of B-arrestin antibody I suggest to use B-arrestin antibody from santa cruz. It gives a single band with immunoblotting.
Date published: 2017-06-26
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of β-Arrestin-2 expression in Hep G2 whole cell lysate.
Date published: 2017-02-01
Rated 5 out of 5 by from Superb immunofluorescence nuclear staining Superb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-18
Rated 5 out of 5 by from Nice Western Blot data of Nice Western Blot data of -Arrestin-2 expression in non-transfected and human -Arrestin-2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-08-27
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