beta-Adaptin Antibody (A-5)recommended for detection of β1-Adaptin and β2-Adaptin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

β-Adaptin Antibody (A-5): sc-74423

β-Adaptin Antibody (A-5) is rated 5.0 out of 5 by 6.
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Datasheets

    • β-Adaptin Antibody (A-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 650-949 of β1-Adaptin of human origin
    • beta-Adaptin Antibody (A-5) is recommended for detection of β1-Adaptin and β2-Adaptin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • beta-Adaptin Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Adaptin (A-5): sc-74423.
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Can β-Adaptin (A-5): sc-74423 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, β-Adaptin (A-5): sc-74423 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from good for immunofluorescence it was used in immunofluorescence 1:100 on primary endothelial cells fixed with PFA
Date published: 2018-05-04
Rated 5 out of 5 by from good antibody The tumor cell samples can be detected well in WB and Co-IP! Good antibody!
Date published: 2017-09-29
Rated 5 out of 5 by from Ottimo funzionamento! Ho ricevuto un'aliquota di prova gratuita un mese fa e sono molto soddisfatta.
Date published: 2017-04-12
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Good specificity in WB in IMR Good specificity in WB in IMR-32 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Robust signal in WB in K Robust signal in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-01-24
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