Anti-beta-Actin Antibody (ACTBD11B7) recommended for detection of β-Actin of mouse, rat and human origin by WB, IP, IF and FCM

Anti-β-Actin Antibody (ACTBD11B7): sc-81178

Anti-β-Actin Antibody (ACTBD11B7) is rated 4.7 out of 5 by 26.
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Datasheets

    • β-Actin Antibody (ACTBD11B7) is a mouse monoclonal IgG1 κ, cited in 884 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to a region near the C-terminus of β-Actin of human origin
    • recommended for detection of β-Actin of mouse, rat and human origin by WB, IP, IF and FCM
    • See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (ACTBD11B7): sc-81178.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for β-Actin Antibody (ACTBD11B7) for WB applications. These reagents are now offered in bundles with β-Actin Antibody (ACTBD11B7) (see ordering information below).
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Does this product have lyophilized powder form,I got a bottle of this product which contains lyophilized powder. The Lot number is G1311. Thank you.

Asked by: wpihub
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Bom dia,  Você poderia me enviar uma cotação formal em PDF deste produto? Atenciosamente, Amanda Souto Machado

Asked by: AmandaS
Thank you for your question. Please check the distributors details for your country on our web site: https://www.scbt.com/scbt/resources/distributors. They will be happy to provide for you the requested quotation.
Answered by: Technical Support Europe
Date published: 2017-06-14

Could I use sc-516132 m-IgG lambda -HRP as a secondary antibody with sc-81178 (b-Actin (ACTBD11B7)?

Asked by: Cary Stelloh
Thank you for question. β-Actin (ACTBD11B7): sc-81178, is a mouse monoclonal IgG1 with a kappa light chain. Therefore, you could not use the mouse IgG lambda light chain binding protein (sc-516132) as a secondary detection reagent with this primary. Instead, you would want to use our m-IgGκ BP-HRP: sc-516102 (https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp) which specifically recognizes the kappa light chain of mouse IgG.
Answered by: Technical Support
Date published: 2017-02-08

Hi, what is the specific type of this antibody? Is this kappa light chain? I need to decide which scondary antibody is to be used agianst this antibody.

Asked by: baizzang1
Thank you for your question. This is a kappa light chain mouse monoclonal antibody. While most anti-mouse IgG secondary antibodies are appropriate for use with this beta-Actin antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-17

What is the recommended secondary reagent for Western blot with this mouse monoclonal primary antibody?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-15

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anonymous
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/beta-actin-antibody-c4
Answered by: Tech Service 6
Date published: 2016-12-15
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Rated 2 out of 5 by from not work well This antibodies does not work well. There are multiple bands.
Date published: 2021-01-02
Rated 5 out of 5 by from 功能很出色,结果很赞 我之前买的试剂,结果非常棒也很特异,希望santa被更多人认可,被更多杂志接收,造福更多科研工作者,也希望未来有更多机会使用santa的产品
Date published: 2020-03-04
Rated 5 out of 5 by from Great antibody I just got it few weeks ago, run my blot with it, and it looks great.
Date published: 2019-05-21
Rated 5 out of 5 by from Great antibody The antibody works great. It almost gives very specific bands.
Date published: 2019-01-18
Rated 5 out of 5 by from Product quality I am working in cancer drug resistance. I took cancer drugs from Sigma but my experience was very bad that drugs were not even working. After that, I bought two drugs erlotinib and Gefitinib from Santa Cruz. Both are really working very nicely.
Date published: 2018-11-20
Rated 4 out of 5 by from Good quality The main band ans weak smaller one at moderate exposition: tested by Western blot and chemiluminesence detection.
Date published: 2018-06-23
Rated 4 out of 5 by from Great product I get a trial package from company. It works good in dilution 1:1000.
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodies I got a free sample from company. I used this antibody 1:1000 on Western blot with protein from endometrial cell lines.
Date published: 2018-05-09
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