Anti-beta-Actin Antibody (AC-15) recommended for detection of β-Actin of broad species origin by WB, IP and IF

Anti-β-Actin Antibody (AC-15): sc-69879

Anti-β-Actin Antibody (AC-15) is rated 4.8 out of 5 by 31.
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Datasheets

    • β-Actin Antibody (AC-15) is a mouse monoclonal IgG1, cited in 920 publications, provided at 100 µg/ml
    • raised against a slightly modified synthetic peptide corresponding to β-cytoplasmic actin
    • recommended for detection of β-Actin of broad species origin by WB, IP and IF; not recommended for detection of adult cardiac and skeletal muscle actins or detection in Dictyostelium discoideum
    • See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (AC-15): sc-69879.
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I didn't see (couldn't find yet) the list of species for which this reacts. Think it would work for molluscs? I need a loading control.....

Asked by: MoHU
Thank you for your question. We do not have a complete list of species reactivity for this β-Actin Antibody (AC-15): sc-69879. We know it is broadly reactive with mammalian and other non-mammalian species, however we are not aware specifically of any publication in mollusks.
Answered by: Technical Support
Date published: 2018-11-19

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Alternatively, SC-47778 is available directly conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-15

I need to detect beta-actin in bovine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from b-actin (sc-69879) b-actin antibody (sc-69879) was working very well in our Western blotting experiments.
Date published: 2021-06-02
Rated 5 out of 5 by from Excellent for WB High sensitivity/specificity for WB in several cell extracts
Date published: 2021-02-10
Rated 3 out of 5 by from work well It works well in WB, but the band is not a single band.
Date published: 2021-01-02
Rated 5 out of 5 by from Strong signal for WB It is very good for WB in JJ012, sw-1353, human chondrosarcoma cells
Date published: 2019-08-05
Rated 5 out of 5 by from β-Actin Antibody (AC-15) β-Actin Antibody (AC-15) is good quality and high stability for western blotting (dilute factor: 1:5000)
Date published: 2019-05-17
Rated 5 out of 5 by from Five stars Worked very well for detecting endogenous beta-actin protein as a loading control in various human cell types by Western blotting.
Date published: 2018-07-25
Rated 5 out of 5 by from Excellent loading control antibody It is really an excellent antibody for loading control. We use 1:1000 dilution in 1% BSA in western blotting for mice heart sample
Date published: 2018-07-12
Rated 4 out of 5 by from Good antibodies It works good in dilution 1:1000 (WB). In detection observed strong bands.
Date published: 2018-06-10
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