Anti-beta-Actin Antibody (4E8H3) recommended for detection of β-Actin of mouse, rat and human origin by WB and ELISA

Anti-β-Actin Antibody (4E8H3): sc-130065

Anti-β-Actin Antibody (4E8H3) is rated 4.2 out of 5 by 5.
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Datasheets

    • β-Actin Antibody (4E8H3) is a mouse monoclonal IgG1 κ, cited in 107 publications, provided at 200 µg/ml
    • raised against a peptide mapping at the N-terminus of β-Actin of human origin
    • recommended for detection of β-Actin of mouse, rat and human origin by WB and ELISA
    • See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for β-Actin Antibody (4E8H3) for WB applications. This reagent is now offered in a bundle with β-Actin Antibody (4E8H3) (see ordering information below).
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Are there any issues with using sc-130065: β-Actin (4E8H3) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-130065 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Great product I used this antibodies for WB in dilution 1:1000. In detection was strong band.
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodies I got free sample of company. I used this antibody 1:1000 on Western blot with 60 ug of protein from tissues and 30 ug of protein from cell lines.
Date published: 2018-05-09
Rated 5 out of 5 by from very good antibody a very good antibody for western blot, nice and sharp band show on the film, highly recommend
Date published: 2017-05-10
Rated 4 out of 5 by from Publishable WB data in a variety of human Publishable WB data in a variety of human cell lines -SCBT Publication Review
Date published: 2014-07-15
Rated 4 out of 5 by from Six tumor cell lines (LnCaP Six tumor cell lines (LnCaP, 22rv1, DU145 and PC3, LAPC-4 and C4-2B) were selected for Western blot experimental studies with this antibody. Publishable results obtained -SCBT Publication Review
Date published: 2014-04-08
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