Anti-Beclin-1/BECN1 Antibody (E-8) recommended for detection of Beclin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Beclin-1/BECN1 Antibody (E-8): sc-48341

Anti-Beclin-1/BECN1 Antibody (E-8) is rated 4.8 out of 5 by 50.
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Datasheets

    • Anti-Beclin-1/BECN1 Antibody (E-8) is a mouse monoclonal IgG2a κ Beclin-1/BECN1 antibody, cited in 152 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 of Beclin 1 of human origin
    • Anti-Beclin-1/BECN1 Antibody (E-8) is recommended for detection of Beclin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Beclin-1/BECN1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Beclin-1/BECN1 (E-8): sc-48341.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Beclin-1/BECN1 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Beclin-1/BECN1 Antibody (E-8) (see ordering information below).

Is this product suitable for use in flow cytometry? Is there a protocol if it is convenient to use? Thanks for help.

Asked by: 1EOS
Thank you for your question. We recommend the AlexaFluor conjugates of this antibody for flow cytometry. You can find our recommended protocol for flow cytometry by following this link: https://www.scbt.com/scbt/de/resources/protocols/flow-cytometry
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

What dilution has worked well with rat brain samples?

Asked by: nybound
Thank you for your question. We do not have any data in rat brain samples specifically when using this mouse monoclonal antibody, BECN1 (E-8): sc-48341. We used this primary at a dilution of 1:200 for most of the Western blot data on our website. We recommend a starting dilution of 1:200 (range of 1:100-1:1,000). A link to our recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-06-14

What is the protocol for immunohistochemistry? I have found the support products, but not the protocol.

Asked by: Diman
Thank you for your question. Here is a link to the IHC protocol: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please feel free to contact Technical Support by phone, email or chat.
Answered by: Tech Service
Date published: 2020-08-11

If I freeze this antibody, will it remain viable for a longer period of time?

Asked by: Trav11
Thank you for your question. We do not run long term storage testing, but warranty all of our products for 1 year from the date of purchase. Freezing the antibody will actually void the warranty as this harms the antibody due to the presence of gelatin in the suspension mixture. The recommended storage temperature is 4ºC and many times the antibody will remain viable under these conditions for much longer than the 1 year warranty date. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 4 out of 5 by from Good results This antibody works good at 1:1000 in colon cancer cell lines
Date published: 2020-12-02
Rated 5 out of 5 by from Good signal in WB HepG2 cells, dilution 1:1000. Strong and clear signal, without specific bands.
Date published: 2020-09-11
Rated 5 out of 5 by from good antibody using 20ug mouse brain lysate it worked well in 1:1000 dilution.
Date published: 2020-07-02
Rated 5 out of 5 by from Good results 1:600 diluted for wb of HepG2 cell lysis, and the band is strong .
Date published: 2018-12-24
Rated 5 out of 5 by from very good antibody for western blotting Dlituion 1:1000 cells: 16:HBE Strong and specific band
Date published: 2018-12-19
Rated 5 out of 5 by from Great antibody I used it with 1:1000 dilution in both human cardiomyocytes and mouse fibroblasts. Got very strong and clear bands.
Date published: 2018-10-10
Rated 5 out of 5 by from really good I tested this antibody this week, the outcome is really great.
Date published: 2018-05-02
Rated 5 out of 5 by from Good All lines are clear and antibody is good,,,,,,,,,,,
Date published: 2018-04-06
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