Anti-Beclin-1/BECN1 Antibody (E-8) is a mouse monoclonal IgG2a κ Beclin-1/BECN1 antibody, cited in 152 publications, provided at 200 µg/ml
raised against amino acids 1-300 of Beclin 1 of human origin
Anti-Beclin-1/BECN1 Antibody (E-8) is recommended for detection of Beclin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Beclin-1/BECN1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Beclin-1/BECN1 (E-8): sc-48341.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Beclin-1/BECN1 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Beclin-1/BECN1 Antibody (E-8) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Beclin 1 Antibody (E-8) is a high quality monoclonal Beclin 1 antibody (also designated BECN1 antibody) suitable for the detection of the Beclin 1 protein of mouse, rat and human origin. Beclin 1 Antibody (E-8) is available as both the non-conjugated anti-Beclin 1 antibody form, as well as multiple conjugated forms of anti-Beclin 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Beclin 1 (BECN1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. Beclin 1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the Ribosomal Protein S6, and both are mediated via amino acids. Beclin 1 expression in various carcinoma cell lines such as MCF7 is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, Beclin 1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, Beclin 1 shares structural similarity to the yeast autophagy gene product, Apg6, and was one of the first mammalian proteins discovered to mediate autophagy.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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