Bax CRISPR Plasmids (h) human Bax-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

Bax CRISPR Plasmids (h)

Bax CRISPR Knockout and Activation Products (h) is rated 5.0 out of 5 by 4.
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Datasheets

    • Target species: human; for corresponding mouse product, see Bax CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of Bax-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using Bax Antibody (B-9): sc-7480
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanBAX58119q13.33XM_017027077, NM_001291428, NM_001291429, NM_001291430, NM_001291431, NM_004324, NM_138761, NM_138763, NM_138764, NM_138762Q07812600040
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Hi, Will Bax activation plasmid work without generating stable expressing clones, the transfection efficiency i achieve is very low to use transfected cells directly. In Bax KO system, even transient expression edits DNA, so there is permanent KO.

Asked by: Siveen K S
Thank you for your inquiry. The activation plasmids will not create a stable cell line overexpressing the gene, it is a transient activation and will not remain in the cells for multiple generations. In KO, you actually alter/edit the gene targeted, which is why there is a permanent knockout. In activation, nothing gets altered/edited, so there will not be a permanent activation. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions.
Answered by: Tech Service
Date published: 2017-11-09
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Rated 5 out of 5 by from We have been very pleased with the activation We have been very pleased with the activation plasmid although the results vary between the different plasmids and from one cell line to the next.
Date published: 2015-05-04
Rated 5 out of 5 by from The activation plasmids give great data depending The activation plasmids give great data depending upon the gene target.
Date published: 2015-04-08
Rated 5 out of 5 by from Western blot analysis verified Bax was upregulated Western blot analysis verified Bax was upregulated in transfected HEK 293T whole cell lysates. -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from Bax upregulation was seen in WB using sc Bax upregulation was seen in WB using sc-23959, single clean band detected in the transfected HEK 293T cells. -SCBT QC
Date published: 2015-03-23
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