Anti-Bax Antibody (YTH6A7) recommended for detection of Bax of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Bax Antibody (YTH6A7): sc-80658

Anti-Bax Antibody (YTH6A7) is rated 4.0 out of 5 by 1.
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Datasheets

    • Bax Antibody (YTH6A7) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
    • raised against the N-terminal residues 12-24 common to human, mouse and rat Bax protein
    • recommended for detection of Bax of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Bax (B-9): sc-7480 for Bax antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Bax Antibody (YTH6A7) for WB and IHC(P) applications. These reagents are now offered in bundles with Bax Antibody (YTH6A7) (see ordering information below).
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Can Bax (YTH6A7): sc-80658 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Bax (YTH6A7): sc-80658 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Satisfactory Western blot analysis of Bax Satisfactory Western blot analysis of Bax expression in non-transfected 293T, mouse Bax transfected 293T and HuT 78 whole cell lysates. - SCBT QC
Date published: 2013-07-29
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