Anti-AVP Antibody (E-8) recommended for detection of AVP precursor, Neurophysin II and copeptin mature chains of mouse origin by WB, IP, IF, IHC(P) and ELISA

Anti-AVP Antibody (E-8): sc-390723

Anti-AVP Antibody (E-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • AVP Antibody (E-8) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 111-160 mapping near the C-terminus of AVP of mouse origin
    • AVP Antibody (E-8) is recommended for detection of AVP precursor, Neurophysin II and copeptin mature chains of mouse origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-AVP Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AVP (E-8): sc-390723.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AVP Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with AVP Antibody (E-8) (see ordering information below).
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Are there any issues with using sc-390723: AVP (E-8) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-390723 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Immunohistochemistry I performed immunohistochemistry (1:50, autoclave) on human tissue samples. It worked.
Date published: 2021-03-17
Rated 5 out of 5 by from Great staining- Tested this as a sample. ran fluorescence immunohistochemistry (1:100) on 50 micron mouse brain slices- perfused with 4% paraformaldehyde
Date published: 2018-11-15
Rated 5 out of 5 by from Excellent AB I used this AB for IF in 4% PFA fixed brains (mouse). Briefly, after blocking the section with 10% NDS in PBST, I incubated the slices with the primary AB, final concentration 1/1000 ON at 4C and then I incubated the samples with a secondary anti-mouse Alexa 594. It worked perfectly even using a low final concentration. The signal is super clear!
Date published: 2018-01-23
Rated 5 out of 5 by from Good Western blot data of AVP expression Good Western blot data of AVP expression in mouse pituitary gland tissue extract. -SCBT QC
Date published: 2014-04-06
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